Week 4: 02/19/2014

Week 4.

Last week, I completed my first DNA extraction on the common bee, then processed an electrophoresis to verify DNA presence. Initially, the EP was unsuccessful, and much time was lost as I focused on the equipment itself as the source of the failure. Further review revealed that my mathematical calculations were in error when I created the buffer solution for use in the EP. As a result the equipment would not conduct the electrical charge. Once the solution was changed to the appropriate percentage of ingredients, the EP was completed and a UV photo was taken. See below. Lesson: Check, then double-check all calculations before proceeding to the next step of an experiment.

 

This week, I am focused on researching universal primers for the identification of bacteria that are commonly stocked in college-level labs. The intent is A) identify usage patterns of primers in existing published research to indicate if universal primers exist, B) to verify the universality of specific primers by replicating results of that existing research, using those common laboratory bacteria, and C) determine if usage of those primers can be adapted into a student lab protocol that will accompany the standard clinical format of bacterial culturing and thereby introduce the student leaner to elementary DNA identification technology.


As I review publications for source material, I will also be learning additional DNA extraction methods. Updates to follow. In the meantime, please enjoy the following: